EFFECT OF MERCURY ON REDUCED GLUTATHIONE OF HUMAN T AND B LYMPHOCYTES: A COMPARISON OF INORGANIC AND ORGANIC MERCURY
Abstract
Material & Methods: The present experimental work was design to analyze the concentration and time dependent effect of mercuric chloride on the chemical modulation of T and B-lymphocytes GSH of human blood. Terasaki technique for lymphocytes isolation and then subsequently separation of T and B-cells was used. Quantification of thiol in T and B-cells was done by Ellman’s method.
Results: The lowest used concentration of mercuric chloride depleted T and B-cells GSH level 2.115 μM (27.34%) and 2.019 μM (32.41%) respectively which was significant (p<0.001) decrease in both T and B-cells GSH level. This effect of mercury was dose and time dependent.
Conclusion: This study suggests that depletion of GSH due to inorganic mercury in T and B-lymphocytes is more severe as compared to its organic form.
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